qscript cdna synthesis kit quantabio (Quanta Biosciences)
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qscript cdna synthesis kit quantabio
Qscript Cdna Synthesis Kit Quantabio, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 3890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qscript+cdna+synthesis+kit+quantabio/qScript+cDNA+Synthesis+Kit/pm41849350-286-181-185
Average 97 stars, based on 3890 article reviews
Qscript Cdna Synthesis Kit Quantabio, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 3890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qscript+cdna+synthesis+kit+quantabio/qScript+cDNA+Synthesis+Kit/pm41849350-286-181-185
Average 97 stars, based on 3890 article reviews
qscript cdna synthesis kit quantabio - by Bioz Stars,
2026-10
97/100 stars
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Multiplex Assay:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Cytotoxicity Assay:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Transcription Factor Assay:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Viability Assay:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 Isolation:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Article Title: Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions. Article Snippet: Article cDNA Synthesis:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Article Title: Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions. Article Snippet: Article Article Title: Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants. Article Snippet: Ethanol 200PRF Decon Labs Cat#V1001 .. Human Luminex Premixed Multi-Analyte Kit Bio-Techne Cat#LXSAHM RNeasy Micro Kit Qiagen Cat#74004 Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 Ii Assay:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Colorimetric Assay:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Microarray:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Gene Expression:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. RNA Sequencing:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Software:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. Article Title: Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions. Article Snippet: Article Indirect Immunoperoxidase Assay:Article Title: The kinase RIPK3 promotes neuronal survival by suppressing excitatory neurotransmission during central nervous system viral infection. Article Snippet: In brief How innate immune signaling within neurons influences neurotransmission remains poorly understood.. Estevez et al. show that the necroptotic kinase RIPK3 promotes neuronal survival during viral infections of the central nervous system by engaging a CaMKII/CREB-dependent transcriptional program that suppresses excitotoxic glutamate signaling.. These findings highlight a mechanism of host protection driven by innate immune control of neurotransmission. other:Article Title: Human papillomavirus E5 suppresses immunity via inhibition of the immunoproteasome and STING pathway. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNAseq data of cell lines This paper GEO: GSE226754 RNAseq data of HPV-positive OPSCCs from Johns Hopkins University cohort Guo et al.21 GEO: GSE112027 Experimental models: Cell lines Human: CAL-27 Dr. J. Silvio Gutkind (University of California, San Diego) RRID: CVCL_1107 Human: HEK293T Dr. Dong-Er Zhang (University of California, San Diego) RRID: CVCL_0063 Mouse: DC2.4 Dr. Dong-Er Zhang (University of California, San Diego) RRID: CVCL_J409 Mouse: MOC2 Dr. J. Silvio Gutkind (University of California, San Diego) RRID: CVCL_ZD33 Mouse: AT-84 Dr. Aldo Venuti (Regina Elena National Cancer Institute, Italy) N/A Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Strain#: 000664; RRID: IMSR_JAX:000664 Mouse: C3H/HeNCrl Charles River Laboratories Strain Code: 025; RRID: IMSR_CRL:025 Oligonucleotides See Table S5 for qPCR primers This paper N/A Recombinant DNA Plasmid: pLXSN-AU1-HPV16 E5 (codonoptimized) Dr. Frank Suprynowicz (Georgetown University Medical School) N/A Plasmid: MIP (MSCV-IRES-Puro) Dr. Dong-Er Zhang (University of California, San Diego) N/A Plasmid: pMSCV-Blasticidin Addgene Plasmid: #75085; RRID: Addgene_75085 Plasmid: pMSCV-Zeo Addgene Plasmid: #75088; RRID: Addgene_75088 Plasmid: pCL-10A1 Novus Cat#: NBP2-29542 Plasmid: pCL-Eco Novus Cat#: NBP2-29540 Plasmid: pcDNATM3.1 (+) Mammalian Expression Vector Thermo Fisher Scientific (Invitrogen) Cat#: V79020 Plasmid: MIP-FLAG-HPV16 E5 (codonoptimized) This paper N/A Plasmid: pMSCV-Blasticidin-HPV16 E6 This paper N/A Plasmid: pMSCV-Zeo-HPV16 E7 This paper N/A Plasmid: pcDNA-FLAG-HPV16 E5 (codonoptimized) This paper N/A Plasmid: pcDNA-HA-HPV16 E5 (codonoptimized) This paper N/A Plasmid: pcDNA-HA-human MAVS This paper N/A Plasmid: pcDNA-human STING-HA This paper N/A Plasmid: pcDNA-human STING-Myc This paper N/A Plasmid: pcDNA-HA-human IRF3 This paper N/A Plasmid: pGL3-Basic Promega Cat#: E1751 Plasmid: pGL3-IFN-b gene promoter This paper N/A Plasmid: pRL-TK Promega Cat#: E2241 Software and algorithms GraphPad Prism 8 GraphPad N/A ImageJ Schneider et al.48 https://imagej.nih.gov/ij/ STAR (v2.6.1) Dobin et al.49 N/A (Continued on next page) Cell Reports 42, 112508, May 30, 2023 17 Article Title: A single-cell CRISPR screen defines a gene regulatory network governing human pluripotency in primed and naive cells. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins mTeSR1 medium STEMCELL Technologies Cat# 05850 Y-27632 Stemgent N/A TrypLE Select Thermo Fisher Scientific Cat# 12563029 Knockout Dulbecco’s Modified Eagle’s Medium (Knockout DMEM) Thermo Fisher Scientific Cat# 10829018 Knockout Serum Replacement (KSR) Thermo Fisher Scientific Cat# 10828028 l-Glutamine Biological Industries Cat# 03-020-1A Nonessential amino acids Biological Industries Cat# 01-340-1B Penicillin and streptomycin Biological Industries Cat# 03-031-1B β-mercaptoethanol Sigma Cat# M7522 Human basic fibroblast growth factor (bFGF) PeproTech Cat# AF-100-18B Fetal bovine serum (FBS) Invitrogen N/A polyethylenimine ‘‘Max’’ (PEI-Max) Polysciences Cat# 24765 Trypsin–EDTA Biological Industries Cat# 03-052-1A Polybrene Sigma Cat# TR-1003 Puromycin dihydrochloride Sigma Cat# P8833 Matrigel hESC-Qualified Matrix Corning Cat# 354277 Neurobasal medium Gibco Cat# 21103049 DMEM/F12 Merck Cat# D6421 N2 Supplement Thermo Fisher Scientific Cat# 17502048 B27 Supplement Thermo Fisher Scientific Cat# 17504044 PD0325901 Sigma Cat# 444968 IM-12 Enzo Life Sciences Cat# BML-WN1020005 SB590885 Cayman Chemical Cat# 16643 WH4–023 Cayman Chemical Cat# 9002067 Activin A PeproTech Cat# 120-14-50 Recombinant human LIF PeproTech Cat# 300-05-100UG Lipofectamine Stem Transfection Reagent Invitrogen Cat# STEM00003 Critical commercial assays RNeasy Micro Kit QIAGEN Cat# 74004 cDNA synthesis:Article Title: Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse CD117 (ckit) (Clone 2B8) - PerCP-Cy5.5 BD Biosciences Cat# 560557; RRID: AB_1645258 Anti-mouse Ly6G (Clone 1A8) - FITC BD Biosciences Cat# 551460; RRID: AB_394207 Anti-mouse CD182 (CXCR2) (Clone SA044G4) - Alexa Fluor 647 Biolegend Cat# 149306; RRID: AB_2565694 Anti-mouse CD184 (CXCR4) (Clone 2B11/CXCR4) - PE BD Biosciences Cat# 551966; RRID: AB_394305 Anti-mouse CD115 (CSF-1R) (Clone AFS598) - Brilliant Violet 605 BD Biosciences Cat# 750892; RRID: AB_2874988 Mouse anti-Ki67 (Clone B56) - PE-Cy7 BD Biosciences Cat# 561283; RRID: AB_10716060 Anti-CD11b (Clone M1/70) - Brilliant Violet 421 BD Biosciences Cat# 562605; RRID: AB_11152949 Anti-mouse CD16/32 (FcgRIII/II) (Clone 2.4G2) - Purified (Mouse BD Fc Block) BD Biosciences Cat# 553142; RRID: AB_394657 Fixable Viability Stain 620 BD Biosciences Cat# 564996; RRID: AB_2869636 Mouse Anti-MPO Antibody (Clone 2C7) Abcam Cat# ab25989; RRID: AB_448948 Rabbit Anti-Neutrophil Elastase antibody Abcam Cat# ab68672; RRID: AB_1658868 Rabbit Anti-Lipocalin-2/NGAL antibody [EPR21092] Abcam Cat #ab216462 Rabbit Anti- MMP-9 Antibody (Clone C-20) Abcam Cat# ab38898; RRID: AB_776512 beta Actin Monoclonal Antibody Invitrogen Cat #AM4302 Rabbit Anti-P62 Novus Biologicals NBP1-48320B Rabbit Anti-PINK1 Novus Biologicals BC100–494 Rabbit Anti-Lamin B2 Abcam ab151735; RRID: AB_2827514 Rabbit p70S6K Cell Signaling 2708; RRID: AB_390722 Rabbit p-p70S6K Cell Signaling 9205; RRID: AB_330944 Rabbit ATG5 Cell Signaling 2630; RRID: AB_2062340 Goat Anti-Mouse Invitrogen 62–6520 mouse anti-rabbit IgG-HRP Santa Cruz Biotechnology Cat# sc-2357; RRID: AB_628497 Bacterial and virus strains LPS (derived from Escherichia coli O55:B5) Sigma-Aldrich Cat# L2880 Salmonella enterica ssp. enterica serovar Typhimurium (SL1344) Brumell lab68 N/A Chemicals, peptides, and recombinant proteins Dihydrorhodamine 123 ThermoFisher Scientific Cat# D23806 DAPI Abcam Cat# 228549 Transcription Factor Buffer Set BD Bioscience Cat# 562725 Brilliant Stain Buffer Plus BD Bioscience Cat# 566385 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# P8139 Trypan Blue Sigma-Aldrich Cat# T8154 TRIzol Reagent ThermoFisher Scientific Cat# 15596018 Collagenase A Sigma-Aldrich Cat# 10103578001 Bovine Serum Albumin Powder Tocris Cat# 5217 Bovine Serum Albumin solution Sigma-Aldrich Cat# A9576 Sodium Chloride Sigma-Aldrich Cat# S9888 RPMI Gibco Cat# 11875093 Advanced qPCR Mastermix Super Green Wisent Cat# 800-431-UL (Continued on next page) 16 Cell Reports 43, 114493, August 27, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER 30% Acrylamide/Bis Solution Bio-Rad Cat# 1610158 CountBright Absolute Counting Beads, for flow cytometry ThermoFisher Scientific Cat# C36950 Tween 20 Sigma-Aldrich Cat# P1379 Seahorse XF RPMI Agilent Cat# 103576-100 Critical commercial assays Direct-zol RNA MiniPrep Kit Zymo Research Cat# R2052 Neutrophil Isolation Kit, mouse Miltenyi Biotec Cat# 130-097-658 Flow Cytometry:Article Title: Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions. Article Snippet: Article Western Blot:Article Title: Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions. Article Snippet: Article Bicinchoninic Acid Protein Assay:Article Title: Mitochondrial perturbations in low-protein-diet-fed mice are associated with altered neutrophil development and effector functions. Article Snippet: Article Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 RNAscope:Article Title: Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants. Article Snippet: Ethanol 200PRF Decon Labs Cat#V1001 .. Human Luminex Premixed Multi-Analyte Kit Bio-Techne Cat#LXSAHM RNeasy Micro Kit Qiagen Cat#74004 Transfection:Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 SYBR Green Assay:Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 Protease Inhibitor:Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 Magnetic Beads:Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 Control:Article Title: USP9X mediates an acute adaptive response to MAPK suppression in pancreatic cancer but creates multiple actionable therapeutic vulnerabilities Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA N-HA-FLAG-pHAGE-GFP-Puro Gift from Wade Harper N/A N-HA-FLAG-pHAGE-MCL1-Puro MCL1 was cloned into the backbone provided by Wade Harper pDONR221 MCL1 Cat#HsCD00042645 pRK5-HA-Ubiquitin-WT Gift from Ted Dawson RRID: Addgene_17608 pLKO.1-shCtrl-Puro Target sequence: 50-CCTAAGGTTAA GTCGCCCTCG-30 Addgene RRID: Addgene_136035 pLKO.1-shUSP9X_1-Puro Target sequence: 50-GAGAGTTTATTCACTGTCTTA-30 Sigma-Aldrich TRCN0000007361 pLKO.1-shUSP9X_2-Puro Target sequence: 5-CGCCTGATTCTTCCAATGAAA-30 Sigma-Aldrich TRCN0000007364 pLKO.1-shUSP9X_3-Puro Target sequence: 50-CACCTCAAACCAAGGATCAA-30 Sigma-Aldrich TRCN0000011091 pLKO.1-shMCL1-Puro Target sequence: 50-GCCTAGTTTATCACCAATAAT-30 Sigma-Aldrich TRCN0000196390 LV05 U6-sgCtrl:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CGCGATAGCGCGAATATATT-30 Sigma-Aldrich Clone ID#NegativeControl1 LV05 U6-sgBIM:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-CTGCAATTGTCTACCTTCT-30 Sigma-Aldrich Clone ID#HSPD0000059472 LV05 U6-sgMCL1:EF1-Cas9+FLAG-2A-Puro Target sequence: 50-GGACCTCGGCGCCAATGGG-30 Sigma-Aldrich CloneID# HSPD0000025257 Software and algorithms GraphPad Prism v9.2.0 GraphPad RRID: SCR_002798 Incucyte S3 Software v2021A Sartorius RRID: SCR_019874 Cancer Dependency Map Portal (DepMap) (https://depmap.org/portal/gene/MCL1?tab= dependency&dependency=Chronos_Combined) Broad Institute RRID: SCR_017655 ImageJ Software v1.5a ImageJ RRID:SCR_003070 SynergyFinder University of Helsinki RRID:SCR_019318 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER AMG-176 (Mcl-1i) ChemieTek Cat#CT-AMG176; CAS#1883727-34-1 EOAI3402143 (USP9Xi) Selleck Chemicals Cat#S6877; CAS#1699750-95-2 MG132 Millipore Sigma Cat#474790; CAS#133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS#66-81-9 THZ1 MedChem Express Cat#HY-80013; CAS#1604810-83-4 THZ531 Gift from Nathanael Gray N/A Staurosporine Sigma-Aldrich Cat#S5921; CAS#62996-74-1 Etoposide Sigma-Aldrich Cat#341205; CAS#33419-42-0 Critical commercial assays Incucyte Nuclight Rapid Red Dye Sartorius Cat#4717 Cell Event Caspase-3/7 Green Detection Reagent Invitrogen Cat#C10423 CellTiter-Glo 3D Cell Viability Assay Promega Cat#PRG9681 Lipofectamine RNAiMAX Transfection Reagent Invitrogen Cat#13778150 x-TremeGENE 9 DNA Transfection Reagent Roche Cat#6365787001 RNeasy Plus Mini Kit Qiagen Cat#74136 Electron Microscopy:Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 Filtration:Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 DNA Purification:Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 Plasmid Preparation:Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 Nuclear Magnetic Resonance:Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 Labeling:Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 Recombinant:Article Title: A unique chaperoning mechanism in class A JDPs recognizes and stabilizes mutant p53. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal (PAb 240) anti-p53 Abcam Cat#ab26; RRID: AB_303198 Mouse monoclonal (DO-1) anti-p53 Santa Cruz Cat#sc-126; RRID: AB_628082 Rabbit monoclonal (EPR8185) anti-Vinculin Abcam Cat#ab129002; RRID: AB_11144129 Rabbit polyclonal anti-DNAJA1 Abcam Cat#ab192904; RRID: AB_3094851 Rabbit monoclonal )SV5-P-K( anti-V5 tag Abcam Cat#ab206566; RRID: AB_2819156 Rabbit monoclonal (DrH8Q) anti-V5 tag Cell Signaling Cat#13202; RRID: AB_2687461 Mouse monoclonal (E10) anti-V5 tag Santa Cruz Cat#sc-81594; RRID: AB_1131162 Rabbit monoclonal (60A8) anti-c-Jun Cell Signaling Cat#9165; RRID: AB_2130165 Mouse monoclonal anti-Actin Invitrogen Cat#MA5-11869; RRID: AB_11004139 Rabbit polyclonal anti-DNAJA2 Invitrogen Cat#PA5-65583; RRID: AB_2662184 Goat anti mouse IgG-HRP JIR Cat#115-035-003; RRID: AB_10015289 Goat anti rabbit IgG-HRP JIR Cat#111-035-003; RRID: AB_2313567 Goat anti-mouse Alexa 568 Invitrogen Cat#A-11004; RRID: AB_2534072 Goat anti-rabbit Alexa 488 Invitrogen Cat#A32731; RRID: AB_2633280 Goat anti-rabbit Alexa Fluor488 Abcam Cat#ab150077; RRID: AB_2630356 Bacterial and virus strains BL21(DE3) Competent Cells Novagen Cat#69450 Rosetta (DE3) Competent Cells Novagen Cat# 70954 Chemicals, peptides, and recombinant proteins Thioflavin T Thermo Scientific Cat#211760050; CAS: 2390-54-7 Luciferase assay system Promega Cat# E1500 MG-132 Cell Signaling Cat#21945S; CAS: 133407-82-6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 DAPI Sigma-Aldrich CAS: 28718-90-3 DAPI Invitrogen Cat#D1306; CAS: 28718-91-4 Lipofectamine 3000 Invitrogen Cat#L3000001 cOmplete Protease Inhibitor Cocktail Roche Cat#11697498001 MDCC Adipogen Cat#CDX-D0198; CAS: 156571-46-9 Insulin Sigma-Aldrich Cat#91077C; GenPept: P01308 Heparin Sigma-Aldrich Cat#H3393; CAS: 9041-08-1 Scrambled RNA Dharmacon Cat# D-001810-10-05 Penicillin-Streptomycin Solution Biological Industries 03-031-1B Alexa Fluor 488 C5 Maleimide Invitrogen Cat# A10254 DMEM Gibco Cat#41965-039 ExpressPlus PAGE GenScript Cat#M42012 NativePAGE Bis-Tris Invitrogen Cat#BN1001BOX ProLong Gold Antifade Invitrogen Cat#P36930 15NH4Cl CIL Cat#NLM-467 13C-D-glucose CIL Cat#CDLM-3813 D-glucose Sigma-Aldrich Cat#552003 Deuterium oxide Sigma-Aldrich Cat#151882 Creatine kinase Sigma-Aldrich Cat#C7886 4-20% gradient SDS-PAGE GenScript Cat#M00656 (Continued on next page) Molecular Cell 84, 1512–1526.e1–e9, April 18, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER ECL Bio-Rad Cat#1705060 Dynabeads protein G Invitrogen Cat#10003D EM grids Electron Microscopy Sciences (EMS) Cat#CF300-CU Uranyl-acetate EMS CAS: 541-09-3 Gel filtration standards Bio-Rad Cat#1511901 p53 b4 peptide (residues 155-164) TRVRAMAIYK GenScript Custom made p53 b9 peptide (residues 248-263) RSPILTIITLEDSSGN GenScript Custom made Critical commercial assays RNeasy Mini Kit Qiagen Cat#74104 iScript cDNA Synthesis Kit Bio-Rad Cat#1708890 TB Green Premix Ex Taq II Takara Cat#RR82WR Wizard Plus SV DNA Purification Minipreps Promega Cat#A1460 |